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Fig. 8 Rab5c variants and dose disturb zebrafish development. Expression of the Rab5c variants was achieved by mRNA microinjection in zebrafish embryos. The different classes and color scheme (A) are defined on developmental parameters such as cephalic development, anterior?posterior axis, caudal edema formation and yolk extension shape. Tissue necrosis and abnormal tail growth are also observed. Class I depicts normal development. Class II displays mild developmental defects observable in the reduced size of the eyes and slight shortening of the AP axis. The yolk extension is short and thick (arrowhead). In class III, the AP axis is visibly shortened, the yolk extension is further shortened and caudal edema may be observed (asterisk). These phenotypes are further exacerbated in class IV; here, head necrosis is also visible. (B) Quantification of the phenotypical classification as described in (A) for the tested Rab5c variants. The y-axis is the percentage of embryos showing each of the four classes. Note the dose?response effect obtained with wt, A31P and I129N variants with increasing mRNA quantities (from 50 pg/embryo to 200 pg/embryo). Injections with S35N and Q80R were carried out at 50 pg/embryo. Scale bars: 100 ?m. DN: dominant negative