Fig. 6 Live imaging of proteins and modified LNPs in the peripheral circulation of 2 days postfertilization larval zebrafish. Modified LNPs (2 nL, approximately 3 × 106 vesicles) or control proteins (2 nL, 30 μM) were injected into the duct of Cuvier and monitored in the heart, in the arterial environment of the dorsal aorta (DA), and in venous vessels in the caudal vein plexus (CVP). Live imaging was performed on a Leica TCS SP8 AOBS confocal laser scanning microscope. GFP is shown in green, and mCherry is shown in red. The images shown are representative of each condition. Scale bars: 50 μm for the heart, 10 μm for DA and CVP images.
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