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Fig. 4 ZnONP exposure induces a local inflammation in close proximity to OS-responding olfactory tissues. (A) Confocal images of neutrophils (green) and mCherry expressing OS responding OSNs (red) in control (top) or 0.1 mg/L (middle) or 1 mg/L ZnONP (bottom) exposed double transgenic Tg(EpRE:mCherry;mpx:GFP) embryo-larvae. (The left panels) The merged images at the final time point of the time-lapse (20 min), (the middle panels) The trajectories of neutrophils in the anterior-brain region (upper half from the dotted line in the image). The trajectories of individual neutrophils are shown with coloured lines. (The right panels) The trajectories of neutrophils in mid-brain regions (lower half from the dotted line in the image). (Bi-iv and Ci-iv) Dot plots for total neutrophil numbers (Bi and Ci, n = 8–9); total distance (Bii and Cii); displacement (start–end distance) (Biii-Ciii); and speed (Biv-Civ) of neutrophil migration during 20 min timelapse imaging are shown. The above parameters in anterior-brain region (Bi-iv); in mid-head region (Ci-iv) are shown. * p < 0.05, ** p < 0.01, **** p < 0.0001 and ns (not significant) by ANOVA with Tukey’s post hoc test using GraphPad Prizm version 9.3.1. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)