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Fig. 4 DNA methylation dynamics during medaka-zebrafish hybrid embryo development. (A) Global mCG/CG levels of the paternal medaka methylome (top) and maternal zebrafish methylome (bottom) in medaka-zebrafish hybrids compared to the closest relative stage in wt zebrafish and medaka. Data is represented as the mean of two biological WGBS replicates with error bars indicating the standard error. (B) Heatmap of mCG/CG levels at developmental DMRs in medaka and the paternal hybrid methylome (left), and zebrafish and the maternal hybrid methylome (right). (C) mCG/CG plotted over developmental ATAC-seq peaks in the paternal medaka methylome (top) and maternal zebrafish methylome (bottom) from medaka-zebrafish hybrids. (D) Heatmap of mCG/CG levels at evolutionarily conserved CTA promoters in medaka and the paternal hybrid methylome (left), and zebrafish and the maternal hybrid methylome (right). Data is represented as the mean of two WGBS biological replicates. (E) IGV browser snapshot of mCG/CG levels during medaka, zebrafish, and medaka-zebrafish hybrid development. Red line = examples of developmental enhancers which normally undergo developmental hypomethylation. Purple line = examples of CTA promoter regions which normally undergo developmental hypermethylation. (F) Heatmap of mCH/CH levels at MoSat-like TGCT-containing tandem repeats in the paternal medaka hybrid methylome (left) and mCH/CH levels at MoSat repeats in the maternal zebrafish hybrid methylome (right).