FIGURE 7

FIGURE 7

Introducing functional piezo1 gene or increasing muscle mass can alleviate scoliosis symptoms. (A) Schematic diagram of rescue plasmid. Notably, sp7/osterix promoter was used to express functional piezo1 gene. (B) Comparison of 3D reconstruction of micro-CT images. (C) Quantification of TMD from 5 individual segments of abdominal vertebrae of wildtype (N = 6), piezo1^{11aa del/11aa del} mutant (N = 8), rescue mutant fish (N = 6), and double mutant of piezo1^{11aa del/11aa del}; mstnb^−/−(N = 5). (D) Combined Cobb angle of piezo1^{11aa del/11aa del} mutant (N = 8) rescue mutant fish (N = 7), and double mutant of piezo1^{11aa del/11aa del}; mstnb^−/− (N = 6). (E) Sagittal section of the spine. Rescue mutant and piezo1^{11aa del/11aa del}; mstnb^−/− have reduced the number of bone with IVD calcification (white arrows). (F) Graph depicting the percentage of bone with IVD calcification across bone segments 9 to 31. Values are presented as mean ± SD and analyzed using one-way ANOVA followed by Tukey’s test. ns = non-significant; **p < 0.01; ***p < 0.001; ****p < 0.0001.