FIGURE 4

FIGURE 4

Detailed analysis of vertebra shape. (A) Comparison of 3D reconstruction of micro-CT images of vertebral bone between wildtype and piezo1^{11aa del/11aa del} at the abdominal part from side and top view. (B) Schematic diagram and quantification of the (C) length ratio (L1/L2) and (D) height ratio (H1/H2) of single vertebrae measurement. About 6 vertebrae from wildtype (N = 5) and mutant (N = 5) were assessed. Length aspect ratio and height aspect ratio were significantly more variable in mutant (p < 0.0001, analyzed using F-test two samples for variances). (E) The micro-CT reconstructions of 3 months zebrafish from both the wildtype and piezo1^{11aa del/11aa del} mutant specimens were performed. The reconstructions were color-enhanced to represent bone density value. To minimize measurement error, trunk or abdominal part was chosen to measure TMD value due to low bone kinks, indicated as white boxes. (F) Measurement of Tissue Mineral Density (TMD). Values are presented as mean ± SD and analyzed using Student’s t-test. **p < 0.01.