ZFIN Image: Rodríguez-Ruiz et al., 2023, Fig. 2

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Figure Caption

Fig. 2 LRRFIP1 and FLII interact with human NLRP1 inflammasome A–F Interaction of NLRP1 with LRRFIP1 and FLII in K562 cells (A, D–F), HEK293 cells (B) and M‐PBMCs (C) assayed by co‐immunoprecipitation assays and PLA (D–F). Endogenous proteins (A, C–F) and transfected FLAG‐tagged full‐length NLRP1, NLRP1ΔCARDΔPYD, NLRP1_S1213A and UPA‐CARD (B) were analyzed. The number of interactions per cell (E) and the percentage of cytosolic interactions (F) are shown (n = 100 cells). Data are shown as the means ± SEM. P values were calculated using one‐way ANOVA and Tukey's multiple range test. n.s., non‐significant; ****P < 0.0001. #, non‐specific band.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ EMBO Mol. Med.