Figure 4

Figure 4

Ltb4r2−/− mice exhibit impaired macrophage migration following LPS-induced inflammation.A, schematic representation of LPS injury model in mice. WT and Ltb4r2−/− mice were injected with lipopolysaccharide (LPS), followed by quantitation of peritoneal macrophage number (by flow cytometry) and number of macrophages infiltrating the liver (by immunohistochemistry). B, representative flow cytometric dot plots of peritoneal F4/80+ cells (macrophages) (left panels) under the indicated conditions and corresponding quantitation (right panel). C, quantitation (from flow cytometry analysis) of F4/80+ and Cd11c+ cells from spleen in WT and Ltb4r2−/− mice following LPS injection. D, representative liver section images of Galectin 3+ macrophages (blue, indicated by arrows) under the indicated conditions (left panels) and quantification of macrophage number in liver tissue (right panel). Scale bar = 100 μm. Data are presented as mean ± SEM; ∗p < 0.05, and each data point represents an independent biological replicate from different animals. For statistics, 1-way ANOVA with Tukey post-test was used for comparisons.