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Fig. 6 Loss of gata2a leads to increased DNA damage and mutation burden in WKM of gata2a?/? MUTs (A and A?) Control irradiation performed at 10 Gy on WKM cells and immunofluorescence against DAPI and ?H2AX. Scale, 25 ?m. (B) WKM from 6 and 12 mpf WT or gata2a?/? adults and immunofluorescence against DAPI and ?H2AX. Imaging is at 40× magnification. (C) Flow cytometry analysis of ?H2AX expression in 12 mpf WKM. p = 0.0296, WT: n = 12 (biological replicates), gata2a?/?: n = 10 (biological replicates). (D) Western blot of ?H2AX and H2A (loading control) in HPC7 cells after 1 ?M NSC348884 (NPM1 inhibitor43) or 100/200 ?M pyrrothiogatain (GATA inhibitor49) treatment for 24 h. (E) Quantification of western blot data, n = 3/condition (biological triplicates). ?p < 0.05, determined by using an unpaired, two-tailed t test in (C). ??p < 0.01 and ?p < 0.05, determined by Friedman test in (E). See also Figure S6.