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Fig. 5 NRT reduces alcohol-induced liver steatosis in zebrafish larvae. (A) Oil-Red O staining was used to detect hepatic lipid accumulation in response to NRT treatment in zebrafish larvae. (B) Quantification of steatosis categorized “normal”, or “steatosis”. The bar chart indicates the percentages of each category in each group. N = 15/groups, three independent experiments. (C) mRNA expression of genes associated with lipogenic transcription factor (srebp1, srebp2, cebpa), lipogenesis (acc1, fasn, dgat2) and lipid uptake (cpt1a, lpl, ldlr). (D) The effect of NRT on EtOH-induced changes of TG level in zebrafish larvae. (n = 15/group, three independent experiments). The data are presented as the mean ± SD; a,b,c mean values with different superscript letters are significantly different among the groups (p < 0.05).