GAH restores BMP signaling and facilitates the repair of vascular impairment induced by DMH1 and DM. Tg (BRE: GFP; kdrl: mCherry) double transgenic embryos were was treated with 10 μM DMH1 (a1–e3) or 10 μM DM (f1–j3) and then treated with GAH as indicated. BMP (green) activity and vascular development (red) and their colocalization were observed by confocal at 48 hpf. Arrows indicated: Se, intersegmental vessel; MsV, mesencephalic vein; PCeV, posterior cerebral vein; CtA, central artery; PCV, posterior cardinal vein; PHS, primary head sinus; VA, ventral aorta; CCV, common cardinal vein. The experiment was repeated twice for each inhibitor. (k,l) Statistical data of BRE and mCherry fluorescent intensity after DMH1 inhibition (a1–e1) and (a2–e2). (m,n) Statistical data of (f1–j1,f2–j2). Compared with the ctr group, p < 0.05 (#), p < 0.01 (##); compared with the 24 h + 10 μM DMH1/DM group, p < 0.05 (*), p < 0.01 (**), and nonsignificant (NS).
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