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Fig. 7 Osteoclast expression and activity in Chi/+. (A) RT-qPCR analysis of acp5a expression at day 0 and 5 dpa revealed a reduced transcript expression in Chi/+ at 5 dpa (n ? 3 pools of six caudal fins for each genotype). (B) TRAP staining of WT and Chi/+ caudal fin samples collected at day 0 and at 5 dpa (the amputation site is indicated by the black dotted line). Osteoclast activity was increased at day 0 and reduced at 5 dpa in mutants respect to WT (n ? 6 caudal fins for each genotype, as indicated by the dots). Scale bar: 500 µm. (C) RT-qPCR analysis of rankl, opg and rankl/opg ratio. No significant differences were detected between genotypes (n ? 4 pools of six caudal fins for each genotype). dpa: days post amputation. In the graphs, each dot represents a single value: circle for WT and square for Chi/+. * P < 0.05, ** P < 0.01, *** P < 0.001, # P < 0.0001.