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Fig. 5 Spike1058 accelerated A?-induced adverse effects in zebrafish embryos. (A) Schematic of the experimental design with zebrafish embryos. (B) Hatching and (C) survival rates of zebrafish embryos induced by A? with or without Spike1058 at 72 hpf. (D) Representative images of healthy, dead, and abnormal embryos. (E) ROS levels of zebrafish embryos induced by A? with or without Spike1058 for 12 h by H2DCFDA. Data were determined by mean ± standard deviation. n = 3 (each replicate contained 8 zebrafish). Statistical analysis was performed through Tukey?s multiple-comparison post hoc test: * p < 0.05, ** p < 0.01, *** p < 0.001 vs normal group; # p < 0.05, ## p < 0.01, ### p < 0.001 vs A? group.