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Fig. 5 Effect of ALKBH5 on cell proliferation and metastasis in vivo. HeLa and CaSki cells were transfected with NC and two siRNAs (si1 and si2) against the ALKBH5 gene, and labeled cells were transplanted into larval zebrafish to observe cell growth and metastasis. (a and b) Representative photos for proliferation (yolk) and metastasis (trunk) of HeLa cells in a zebrafish model taken by confocal laser scanning microscope are shown (scale bar: 100 μm). (c-f) The fluorescence of HeLa and CaSki cells in different sites of zebrafish was visualized by stereomicroscope and quantified using Image J software and normalized by negative control (n = 12). NC, negative control.