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Fig. 4 tek MO injections into the zebrafish tekbns399 RNA-less allele cause vascular phenotypes. (A-H) Lateral views of trunk vessels in 22 hpf tekbns399/+ incrossed embryos injected with 2 (A,B), 4 (C,D), 6 (E,F) or 8 (G,H) ng of tek ATG1 MO. (I) Total length of 10 intersegmental vessels (ISVs) above the yolk extension in 22 hpf tekbns399/+ incrossed embryos injected with 2, 4, 6 or 8 ng of tek ATG1 MO. (J-Q) Lateral views of trunk vessels in 48 hpf tekbns399/+ incrossed embryos injected with 2 (J,K), 4 (L,M), 6 (N,O) or 8 (P,Q) ng of tek ATG1 MO. Red arrowheads indicate abnormal ISV formation; red asterisks indicate absent ISVs. (R) Number of defective ISVs above the yolk extension in 48 hpf tekbns399/+ incrossed embryos injected with 2, 4, 6 or 8 ng of tek ATG1 MO. In I and R, data are mean±s.d. [by one-way analysis of variance (ANOVA) followed by Tukey's HSD test]. Scale bars: 200 μm.