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Fig. 5 In Mci mutants, the DD pathway for basal body production is strongly affected but not the MCD pathway. (A) ALI cultured wild-type MCCs showing DEUP1-positive deuterosomes. (B) Display of only DEUP1 staining from A, showing deuterosomes (arrows). (C) ALI-cultured Mci mutant airway cells showing DEUP1-positive deuterosomes. (D) Display of only DEUP1 staining from C, showing a deuterosome (arrow). Scale bars: 5 μm. (E) Quantification of the numbers of DEUP1+ deuterosomes in differentiating wild-type and Mci mutant MCCs under ALI conditions. Twenty cells were counted for each genotype at ALI day 3. ****P≤0.0001. (F-I) RT-qPCR analysis of MCD pathway gene expression levels between wild-type and Mci mutant airway cells in ALI culture. Relative expression levels are plotted on the y-axis, and days in ALI culture on the x-axis. Data are mean±s.e.m. Analysis was carried out on three independent biological replicates.