ZFIN Image: Dai et al., 2022, Fig. 1

Image

Figure Caption

Fig. 1 Csf1rb is highly expressed in hemogenic endothelium and HSPCs. (A,B) Diagram at the top shows a 30-36 hpf zebrafish embryo; the orange line indicates the VDA region. (A) WISH of csf1rb in the Tg(flk1:GFP) line at 36 hpf in the VDA region. White arrows indicate colocalization of csf1rb (red) and GFP+ HE (green) with a flat shape. Yellow arrowheads indicate csf1rb-positive naïve HSPCs with a round shape. n=12. (B) WISH of csf1rb in the Tg(cmyb:GFP) line at 30 hpf in the VDA region. Yellow arrowheads indicate colocalization of csf1rb (red) and GFP+ HSPCs (green). White arrowheads indicate cmyb-GFP+ multiciliated cells. n=12. (C,D) Diagram at the top shows a 2 dpf zebrafish embryo; the orange line indicates the VDA region. (C) WISH of csf1rb in the VDA of Tg(cmyb:GFP) at 2 dpf. White arrowheads indicate cmyb-GFP+ multiciliated cells. n=12. (D) WISH of csf1rb in the VDA of Tg(runx1:en-GFP) at 2 dpf. Yellow arrowheads indicate colocalization of csf1rb (red) and GFP+ HSPCs (green). n=15. Brightfield images are two to five layers stacked to show the VDA region (indicated by the red dashed lines). Scale bars: 20 μm.

Figure Data

Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development