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Fig. 6 The Pcdh18a cytoplasmic domain is required for HSPC lodgment and expansion in the CHT. (A) Whole-mount in situ hybridization for myb expression on control or pcdh18a-ΔCP106 morphant embryos at the indicated developmental stages. Arrows indicate myb-expressing cells. (Ba-Bd) Confocal maximum projections of control or pcdh18a MO-injected Tg(CD41:GFP; kdrl:ras-mCherry) embryos at 48 hpf (Ba,Bb) and larvae at 5 dpf (Bc,Bd). ca, caudal artery; cv, caudal vein; isv, inter-somitic vessel. (C) Counts of CD41:GFPlow HSPCs and CD41:GFPhigh pro-thrombocytes in the CHT over a nine-somite width at 48, 58 and 68 hpf (n=6 and 5 larvae for control and pcdh18a morphants, respectively, pooled from three independent experiments), monitored from time-lapse imaging sequences starting at 48 hpf for 20 h. Scale bars: 100 µm.