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Fig. 7 Adult gfi1aasmu10 mutants display hematological disorders. (A) The neutrophil progenitor portion is increased in 10-month-old adult gfi1aasmu10 mutants. Sorted neutrophil lineage cells from the KM of 10-month-old Tg(mpx:GFP);WT and Tg(mpx:GFP);gfi1aasmu10 mutants were subjected to May-Grünwald-Giemsa staining and separated into N1, N2 and N3 groups according to their morphology. *P<0.05 (Mann–Whitney test, n=7). Data are mean±s.e.m. (B) The neutrophil progenitor proportion is increased, whereas the well-differentiated neutrophil portion is decreased in 14-month-old adult gfi1aasmu10 mutants. *P<0.05, **P<0.01 (Mann–Whitney test, n=3). Data are mean±s.e.m. (C,D) Blood cell counts of KM (C; *P<0.05, Student's t-test, n=8) and PB (D, n=9) in 10-month-old fish were determined manually according to their morphology. Data are mean±s.e.m. (E,F) Blood cell counts of KM (E; *P<0.05, Student's t-test, n=10) and PB (E; *P<0.05, **P<0.01, ***P<0.001, Student's t-test and Mann–Whitney test, n=9) in 18-month-old fish were determined manually according to their morphology. Data are mean±s.e.m.