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Fig. 3 Effect of hypoxia on survival, cell death, and gene expression in (stim2a;stim2b)+/+ and (stim2a;stim2b)−/− larvae. (A) The survival rate is expressed as the mean ± SEM of n ≥ 180 larvae per group. (B) On the left upper panel, there are representative images of AO staining and the lower panel presents AO-positive cells in the OT in (stim2a;stim2b)+/+ and (stim2a;stim2b)−/− larvae. The right panel shows its quantification, n = 18 larvae per group. (C) Expression of selected genes that are related to cell death pathways and (D) microglia-associated genes, estimated by qPCR, in (stim2a;stim2b)+/+ and (stim2a;stim2b)−/− larvae. The data are expressed as the mean ± SEM of n = 3–6 samples/group. *p < 0.05, **p < 0.01, ***p < 0.001. AO, Acridine Orange; OT, optic tectum; qPCR, quantitative polymerase chain reaction.