Figure 7.
- ID
- ZDB-IMAGE-231212-48
- Publication
- Singh et al., 2023 - NMJ analyser: a novel method to quantify neuromuscular junction morphology in zebrafish
- All Figures
- Figures for Singh et al., 2023
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Figure 7.
Co-localization analysis. Co-localization is based on percent overlap. (A and B) A Boolean “AND” operation is performed pixel-wise across the two channels of an image, resulting in an image where only pixels that were common to both channels remain white. The program then calculates the area in each punctum taken up by white pixels. A punctum is classified as “single” if the fraction of white pixels falls below the co-localization threshold and “colocalized” if the fraction is above the threshold. (C) The program tabulates the number of “single” puncta, “colocalized” puncta, sorted according to different thresholds from >0% to =100%.