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Figure 4.

ID
ZDB-IMAGE-231212-45
Publication
Singh et al., 2023 - NMJ analyser: a novel method to quantify neuromuscular junction morphology in zebrafish
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Figures for Singh et al., 2023
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Figure 4.

Image processing and Otsu thresholding for puncta detection. The original image was subjected to a Gaussian blur and contrast enhancement algorithm to enable the detection of puncta via Otsu thresholding. However, the limitations in resolution posed challenges in properly separating clustered puncta. The lack of resolution limits the clustered puncta to be separated. Dashed circle represents the clustered puncta; solid circle represents the single punctum. Scale bar = 2.5 µm.

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