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Figure Caption
Fig. 1 Screenshot of the UCSC Genome Browser with CRISPRscan and ZebrafishGenomics hubs enabled to identify sgRNA target sites. Use tracks (marked in yellow) to search for targets that can be synthesized with a T7 promoter (guide sequence begins with a “GG”) as close as possible to the intended nucleotide for point mutation or site of insertion. In this example, both CRISPRscan and ZebrafishGenomics predicted two overlapping targets (sgRNA-T1 and sgRNA-T2) close to the target nucleotide (marked in red box) and were selected for sgRNA synthesis.
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