Fig. 3

Fig. 3 Impaired motor behaviour and exercise-induced mortality in zebrafish Dsp mutant larvae.

A–A′–A″: Birefringence analysis on WT and mutant larvae at 5 dpf does not detect any significant difference in the skeletal muscles structure. All embryos are at 5 dpf, in lateral view, anterior to the left. Sample size: WT n = 20; -ab n = 30. R.I. relative intensity. Error bars: SEM. ns not significant. Test: Unpaired t-test. B–B′: WT and -ab larvae at 5 dpf display normal response to light (white areas) and dark (grey areas) stimuli, but with reduced motor performances if mutated, evaluated as total distance swum (B). Sample size: n = 36. Error bars: SEM. *p < 0.05. Test: Unpaired t-test. C A mild training protocol was applied on larvae from 3 to 13 dpf, for 10 days. A statistically significant increase in mortality was observed in trained mutants when compared with resting controls (p < 0.0001). Sample size: n = 50. Error bars: SEM. Test: Log-rank (Mantel–Cox) test.