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Fig. 1.

ID: ZDB-IMAGE-231202-2
Publication: Zlatanova et al., 2023 - An injury-responsive mmp14b enhancer is required for heart regeneration
All Figures: Figures for Zlatanova et al., 2023

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Figure Caption

Fig. 1. mmp14b expression is induced in endothelial cells in response to heart injury in zebrafish.

(A) Experimental design for heart injury and subsequent ATAC-seq from cardiac ventricle endothelial cells. (B) Genome browser view showing ATAC-seq peak (enh) at 3 dpa in the mmp14b gene in uninjured (Ctl) and injured (Amp) eGFP⁺ cells. (C) RNAscope in situ hybridization for mmp14b expression in wild-type adult heart sections from uninjured (sham) and injured (7 dpa) hearts. mmp14b expression (red fluorescence) is evident in the injured area (dashed line). (D) Relative expression of mmp14b in wild-type zebrafish hearts expressed in arbitrary units (AU) without injury (sham) in white and injured (Amp) in gray at 1, 3, 7, 14, and 30 dpa as measured by qPCR. DAPI, 4′,6-diamidino-2-phenylindole; A, atrium; V, ventricle; BA, bulbous arteriosus. **P < 0.01.

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