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Figure 5

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ZDB-IMAGE-231127-87
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Figures for Reinhardt et al., 2023
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Figure 5

Dissemination of MDA-MB-231 cells spiked into a DLA sample after injection with the DanioCTC workflow. Cell localization was monitored at 1 and 3 dpi. (A) Representative image of 50 MDA-MB-231 cells injected into the DoC of a zebrafish embryo (1 hpi). (B) Representative image of the tail and CHT region at 3 dpi with localized MDA-MB-231 cells in the CHT (black arrowheads). (C) Representative image of the tail region at 3 dpi showing extravasated MDA-MB-231 cells (black arrowheads). (D) Absolute MDA-MB-231 cell numbers detected in the head, trunk and tail regions at 1 and 3 dpi (n = 11 embryos). Green label: endothelium; red label: MDA-MB 231 cells. dpi: days post injection. Kruskal–Wallis test followed by post hoc Dunn’s test, * 0.01 < p < 0.05; ** 0.001 < p < 0.01.

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