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Figure Caption
Fig. 4 (a) Schematic representation of creation of humanised zebrafish lines. (b) 5 dpf larval zebrafish treated with PTU. Left, rab28-/- non transgenic larvae. Right, rab28-/- larvae transgenically expressing GFP tagged human RAB28. Arrows show the left eye of each larva. (c) Expression vectors used to create RAB28 humanised zebrafish lines. Multiple sequence alignments showing identical amino acids in all model organisms for site 23 and 217. S23F and C217W mutations mapped to RAB28 isoform2 protein domains.
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Reprinted from Vision Research, 210, Moran, A.L., Fehilly, J.D., Blacque, O., Kennedy, B.N., Gene therapy for RAB28: What can we learn from zebrafish?, 108270108270, Copyright (2023) with permission from Elsevier. Full text @ Vision Res.