ZFIN Image: Hall et al., 2023, Figure 1

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Figure Caption

Figure 1

Generation and classification of the Mgat1b^−/− mutant line. Depiction of N-glycan types and the sequential processing initiated via GnT-I (Mgat1) from the simplest N-glycan type (oligomannose) to more processed hybrid and complex types. The area encased within the dashed red box indicates the common pentasaccharide core (A). Products of BclI digest, which yield either homozygous Mgat1b^−/−, heterozygous Mgat1b⁺^/−, or Wt AB (Mgat1b^+/+). Number adjacent to Kb ladder (500 bp) indicates band size (B). Chromatogram with coding sequence of a fragment of Mgat1b from a Wt AB fish compared to that of Mgat1b^−/−, revealing five nucleotide deletions (Δ5), which generated a premature stop codon (encased in red box) (C).

Acknowledgments

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