Fig. 5.

Fig. 5. csf1a and il34 are detected in distinct follicle cell populations of the early ovary.

(A to C) UMAP plot of indicated gene’s expression in the 40-dpf ovary in (A) and (B) follicle cells, and (C) reclustered subpopulation of pre-follicle cells. Subpopulation of pre-F cells boxed in (A) and (B). UMAP of reclustered pre-F cells; legend boxed in (C). (D) Gene expression correlation values of indicated genes in specified clusters of ovarian cells represented by Spearman’s Rho correlation analysis. (E and G to I) Double HCR RNA FISH maximum projections of confocal images of 40-dpf bmp15 (E) heterozygous and (G) to (I) mutant ovaries. il34, red; csf1a, yellow; and DAPI (DNA), cyan. Asterisk (*) indicates blood cells. Regions boxed in (G) are magnified in (H) and (I). Scale bars, 10 μm. (F) Analysis of expression profiles of indicated genes in specified clusters of follicle cells represented by dot plot graph. MAFCs, macrophage-activating follicle cells.