ZFIN Image: Wu et al., 2023, Fig. 7

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Figure Caption

Fig. 7 Expression of gdf9 and bmp15 genes in PG and PV follicles of nobox−/−;dmrt1−/− double mutant at 100 dpf.

Total RNA was extracted from the isolated PG and PV follicles and reverse transcribed into cDNA for real-time PCR analysis. Each data point represents PG or PV follicles isolated and pooled from two fish for each genotype, totaling 10 fish (n = 5 independent samples). The mRNA levels of each target gene were normalized to that of the housekeeping gene ef1a, and expressed as a fold change relative to the control. Data shown are mean ± SEM, P values revealed by one-way ANOVA and Tukey’s test.

Acknowledgments

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