Fig. 1

Fig. 1 Phenotype analysis of different genotypes of figla and dmrt1 mutations at 60 dpf.

a Gonads and secondary sex characteristics of four different genotypes: normal ovary and testis in the control (figla+/−;dmrt1+/−; n = 15 independent fish, 7 males, 8 females); normal ovary and underdeveloped testis in dmrt1 single mutant (figla+/−;dmrt1−/−; n = 16 independent fish, 2 males, 14 females); all-male testis in figla single mutant (figla−/−;dmrt+/−; n = 13 independent fish, 13 males) and underdeveloped testis in figla and dmrt1 double mutant (figla−/−;dmrt1−/−; n = 15 independent fish). Asterisk, breeding tubercles on pectoral fins; arrowhead, genital papilla. b Sex ratio in four different genotypes at 60 dpf. The sample sizes for independent fish are shown at the top of the columns. PG primary growth, PV previtellogenic, EV early vitellogenic, me meiotic cells, sc spermatocytes, sg spermatogonia, sz spermatozoa.