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Figure Caption
Fig. 3 Multiple sequence alignment of the catalytic domains of IYD homologs. Alignment of homologs chosen for protein expression was generated using MUSCLE.45 Numbering of the amino acids for each protein is indicated on the left and right of the alignment. Residues in red (or white) are fully conserved, and the sequences forming the active site lids are indicated with a box. Key residues coordinating to substrate and FMN are indicated with an (*) and (+), respectively. Secondary structure elements are derived from the crystal structure of mmIYD bound to DIT (PDB ID 3GH8).11
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