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Fig 3
The effects of six plant extracts on blood vessel formation in SIVs of
(A) Hemorrhage (white arrow) was observed in 3 dpf zebrafish larvae treated with KT14 150 μg/mL. (B) Microscopic view of blood vessel formation of zebrafish at 72 hpf in the absence and presence of ectopic angiogenesis of Sub intestinal vessels (SIVs), SIVs are marked by a red frame and shown at a higher magnification. The number of “+” is the number of sprouts projecting from the SIVs basket. (C) Quantitative analysis of the percentage of each angiogenic phenotype in SIVs after treatment with the different plant extracts. Sample groups were assessed according to “+”, “++”, “+++”, “++++” and “+++++” representing the percentage of embryos with one, two, three, four or five sprouts projecting out of the SIV regions, respectively. Images were taken using ZEN software. Data present mean ± SD from three independent experiments. Statistical analysis was performed using a two-way ANOVA test, p-values < 0.05 *; 0.01 **; 0.001***.