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ID: ZDB-IMAGE-231107-32
Publication: Demers et al., 2022 - PEX13 prevents pexophagy by regulating ubiquitinated PEX5 and peroxisomal ROS
All Figures: Figures for Demers et al., 2022

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Figure Caption

Fig. 1 Depletion of PEX13 results in decreased peroxisome numbers. (A) HeLa cells were transfected with siRNA against PEX1, PEX2, PEX3, PEX5, PEX6, PEX10, PEX12, PEX13, PEX14, PEX19, PEX26 as well as a non-targeting control siRNA (siCTRL). Cells were fixed and immunostained for endogenous peroxisome marker ABCD3. Scale bar: 25 μm (B) Graph of ABCD3 density of images in (A). (n = 3; 30 cells/trial). Error bars represent standard deviation. (C) HeLa cells treated with siCTRL or siPEX13, overexpression WT PEX13-MYC, W313G or I326T, stained anti-MYC and anti-ABCD3. Scale bar: 10 μm. (D) Quantification of images in (C) (n = 3; >30 cells/trial). Error bars represent standard deviation. Asterisks denote p-values compared to siCTRL: *p < 0.05, **p < 0.01.

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