Fig 1

Fig 1 The effect of cilostazol on the developing zebrafish at various stages.

The wild-type embryos of zebrafish were treated with increasing concentrations of cilostazol as well as the blank or vehicle controls from 10 hpf onwards. For each treatment group, 10 embryos were subject to the oblique illumination microscopy and measurement of heart rate by ImageJ TSA method at 31 hpf, 2 dpf and 3 dpf respectively. Histograms with different letters above them are significantly different (ANOVA and Tukey’s multiple comparisons test, P < 0.05). The representative video clips for the heartbeat of 3 dpf embryos in the blank control, vehicle control or 100 μM cilostazol-treated groups are shown in S1, S2 and S3 Videos respectively.