ZFIN Image: Kim et al., 2023, Figure 2

Image

Figure Caption

Figure 2

PPARα activation reduces YAP signaling in Tg(fabp10a:pt-β-catenin) livers. (A) Heatmap showing the differentially expressed hepatocyte, BEC, and YAP-related genes between DMSO- and GW7647-treated groups. Each column represents an independent sample (n = 3). (B) Upstream analysis showing the top 10 significantly upregulated gene sets of transcriptional regulators in the GW7647-treated group. (C) Pathway analysis showing biological pathways significantly induced in the GW7647-treated group. (D) Immunofluorescence images showing the expression of hCCN2:GFP and Anxa4 in control and Tg(fabp10a:pt-β-catenin) livers at 14 dpf. Dashed lines outline the livers. (E) qRT-PCR data showing the relative expression levels of two YAP target genes (ccn1, ccn2) and yap1 between DMSO- and GW7647-treated Tg(fabp10a:pt-β-catenin) livers at 14 dpf. **P < 0.01; statistical significance was calculated using an unpaired two-tailed t-test. (F) Immunohistochemistry data showing Yap1 expression in 14-dpf Tg(fabp10a:pt-β-catenin) livers treated with GW7647. Scale bars, 50 μm.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.