Fig 7

Fig 7 Rod cell regeneration is enhanced in txn, stat5a/b and c7b crispants.

(a) Experimental design depicting CRISPR/Cas9-based targeting of target genes via CRISPR/Cas9 in NTR-rod embryos. Control (+Mtz) and mutated NTR-rod larvae (+Mtz, gene target MUT) were treated with 10mM Mtz from 5–6 dpf and rod fluorescence was assessed via plate reader assay at 9 dpf (to examine the extent of rod cell regeneration). Created with Biorender.com. (b) Quantification of rod cell regeneration by plate reader assay at 9 dpf in Mtz-treated control and MUT larvae for target genes pparg, prdm1a, nfia, txn, stat5a/b and c7b. (c) Representative in vivo confocal images of regenerated rod cells at 9 dpf in control (+Mtz) and stat5a/b or c7b MUT larvae. For statistical comparisons, Welch’s one-way ANOVA was followed by student’s t test with Dunnett’s method for multiple comparisons correction. Asterisks indicate the following p-value ranges: * = p<0.05, ** = p<0.01, *** = p<0.001, and **** = p<0.0001, “ns” indicates p>0.05.