Figure 1

Figure 1

Expression analyses of clic5 in zebrafish. (A) Semi-quantitative reverse-transcription polymerase chain reaction (RT-PCR) on cDNA of different adult zebrafish organs revealed expression of clic5a (223 bp) in the spleen and of clic5b (408 bp) in the gut, kidney and liver. H₂O served as negative control and ef1α as loading control; Primer dimer (PD). (B) Whole-mount in situ hybridization (WISH) analysis reveals expression of clic5 in the pronephric tubules (black arrow) at 1 day post-fertilization (dpf) which persisted throughout the first days of zebrafish development. At 2dpf, clic5 was additionally expressed in the eye lenses (black arrowheads), liver primordium (white arrowhead), pectoral fin buds and glomerulus (white arrows). At 5dpf clic5 expression was detectable in the liver (white arrowhead) and gut (black arrowheads) (framed box shows the expression of clic5 in the liver and gut from the dorsal view). WISH analysis with specific probes for clic5a and clic5b reveals expression of clic5a in the eye lenses and glomerulus at 2dpf and expression of clic5b in the pronephric tubules at 1 and 2dpf with additional expression in the pectoral fin buds and liver primordium at 2dpf.