ZFIN Image: Torraca et al., 2023, Figure 2.

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Figure 2.

Persistent Shigella represents a clonally expanded population. mCherry- and GFP-labeled S. sonnei 53G strains were coinjected at a 1:1 ratio. A, At 0 hpi, most larvae (approximately 90%) displayed an expected 1:1 ratio of the coinjected strains (ie, percentage difference between strains ≤20%). B, At 144 hpi, most larvae (approximately 80%) were infected by 1 of the 2 strains at a much higher frequency than the other strain (ie, percentage difference between strains >80%), indicating the establishment of clonality. Statistics: differences in distribution between data at 0 and 144 hpi were calculated by chi-square test. ****P < .0001. hpi, hours postinfection.

Acknowledgments

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