ZFIN Image: Ali et al., 2023, Fig. 1.

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Figure Caption

Fig. 1.

Generation of pkd1l1^hsc117 zebrafish mutants. (A) Pkd1l1 is a large membrane-spanning protein with multiple transmembrane domains, with a predicted length of 2153 amino acids (England et al., 2017). The asterix indicates the CRISPR/Cas9 deletion target site. (B) Pairing of nucleotide and amino acid sequences of wild-type and CRISPR/Cas9-derived mutants, revealing mutant F0 zebrafish with a 4 bp deletion, resulting in a premature stop codon. (C,D) F1 embryos were raised, heterozygotes identified and F1 heterozygotes were incrossed to generate pkd1l1^hsc117 mutant embryos. At 5 dpf, wild-type (C) and pkd1l1^hsc117 (D) zebrafish are morphologically indistinguishable. Images are representative of 50 fish (n=50). Scale bars: 1 mm.

Acknowledgments

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