|
Fig. 12 Induction of trained immunity in neutrophils and M1-macrophages through the vaccination of zebrafish with inactivated SVCV. (A) tSNE nonlinear clustering was used to visualize the percentages of neutrophils in the PBS-administered (control), SVCV-infected (infected), and SVCV-vaccinated plus SVCV-infected (vaccinated+SVCV) groups. (B) Histogram showing the differences in neutrophil percentages among PBS-administered, SVCV-infected, and vaccinated+SVCV groups. (C) tSNE nonlinear clustering was used to visualize the percentages of M1-macrophages in PBS-administered, SVCV-infected, and vaccinated+SVCV groups. (D) Histogram of different M1-macrophage percentages in PBS-administered, SVCV-infected, and vaccinated+SVCV groups. (E) Heatmap of important immune genes in neutrophils that are differentially expressed among the PBS-administered, SVCV-infected, vaccinated+SVCV groups. (F) Heatmap showing the important immune genes that are differentially expressed in M1-macrophages among the PBS-administered, SVCV-infected, and vaccinated+SVCV groups.