The bmp4 expression was increased after virus or poly(I:C) challenge. (A) Distribution of bmp4 mRNA in different tissues of adult zebrafish. (B) The expression of bmp4 in the gill, liver, spleen, kidney and intestine from zebrafish challenged with GCRV. Zebrafish injected i.p. with MEM were used as the control. (C,D) Expression of bmp4 mRNA in the ZFL cells challenged with poly(I:C) (C) or GCRV virus (D). ZFL cells challenged with PBS (C) or MEM (D) were used as the control. The expression of actb1 served as an internal control for the qRT-PCR. Data were from three independent experiments and were analyzed through Student’s t-test (two-tailed). All data were presented as mean ± SD (** p < 0.01, *** p < 0.001).
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