Figure 3

Figure 3

opn5 is expressed in bipolar and amacrine cells in the INL and in RGCs. (A,C) FISH against opn5 (red) followed by immunohistochemistry against a bipolar cell marker (Otx2; green) (A) or an amacrine and retinal ganglion cell marker (Pax6; green) (C), on stage 41 Xenopus laevis transverse retinal sections. DAPI (blue) stains the nucleus. ONL (outer nuclear layer), INL (inner nuclear layer), and GCL (ganglion cell layer) are indicated between dotted lines. White arrows point to opn5-expressing cells that are either Otx2⁻ or Pax6⁻, while yellow arrows indicate opn5⁺ cells that are also Otx2⁺ or Pax6⁺. RPE, retinal pigment epithelium. Scale bar = 50 μm. (B,D,E) Graphs showing percentage of opn5⁺ cells that are Otx2⁺(B) or Pax6⁺(D), and the distribution of Pax6⁺/opn5⁺cells between the INL and GCL of stage 41 retina (E). Each dot represents the percentage of cells in one central retina section [(B), n = 6 sections; (D,E), n = 7 sections]. Lines are the mean with 95% confidence interval.