ZFIN Image: Zhao et al., 2023, FIGURE 2

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Figure Caption

FIGURE 2

The spatial and temporal expression patterns of zebrafish Pin1. (A) Semiquantitative RT-PCR analysis of pin1 expression in different stages of zebrafish development. β-actin served as an internal control. (B) Western blot analysis of Pin1 expression in adult zebrafish tissues. Coomassie Blue staining served as internal control. Each lane contains 30 µg of total protein. (C–F) WISH analysis of pin1 expression in 14 hpf embryo (C), 24 hpf (D) and 48 hpf embryo (E,F). Arrow in (E) indicate midbrain-hindbrain boundary (MHB). Arrows in (F) mark the neuromasts staining in the PLL system.

Acknowledgments

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