Fig. 5

Fig. 5 Immune response to ligament transection injury.

UMAPs of single-cell RNA sequencing datasets colored by cluster (a) and injury sample (b). c Dot Plot showing marker gene expression for each immune cluster. Repeated live imaging of neutrophils (d; mpx:mCherry) (n = 5) and macrophages (e; mpeg1.1:eGFP+) (n = 6) at the injury site (arrowheads) during the first week of regeneration color-coded by signal intensity. f Dot Plot demonstrating fn1a expression in macrophage cluster cells split by Sample and Feature Plot for 1 dplt-macrophage enriched gene fn1a. g Representative images of smFISH staining using fn1a (green) and mpeg1.1 (magenta) probes in IOM ligaments from uninjured and 1 dplt samples (n = 3). h Feature Plots for macrophage marker mpeg1.1 and macrophage-enriched gene lgmn. i smFISH for lgmn (yellow) and mpeg1.1 (magenta) probes in IOM ligaments from uninjured and 1 dplt samples (n = 3). Gray dashed lines outline the IOM ligament and inset positions are denoted by the numbers 1-2 (g, i). Scale bars = 500 μm (d, e), 50 μm (g, i).