Fig. 3

Fig. 3 Single-cell transcriptomics of joint tissue through early ligament regeneration.

a Schematic detailing the generation and analysis of single-cell RNA sequencing data. Jaw joints from uninjured, 1 day post-SHAM (nicking of the epithelium above the ligament) surgery, 3 days post-SHAM surgery, 1 day post-ligament transection, and 3 days post-ligament transection were dissociated and sorted for DsRed (neural crest-derived) or BFP (non-neural crest-derived). b UMAP of low-resolution superclusters for merged samples. Superclusters include 2 neural crest-derived clusters (NC-Derived), 2 immune clusters, 3 epithelial clusters, and 1 endothelial cluster. c UMAP colored for neural crest-lineage enrichment. Cells from FACS-sorted DsRed+ neural crest-derived cells colored in red, cells from sorted BFP+ non-neural crest-derived cells colored in blue. d DotPlot detailing expression of marker genes for each supercluster.