Fig 6

Fig 6 Rescue of vitellogenic growth in bmp15-/- females by E2 treatments.

(A) Histology analysis of the bmp15-/- ovary after E2 treatment. The mutant fish were treated from 80 to 100 dpf by E2 via water-borne exposure (10 nM) or oral administration by feeding (2, 20 and 200 μg/g diet). All five fish fed with E2-containing diet at 2 μg/g resumed vitellogenic growth with yolk mass (asterisk) whereas some fish at 20 μg/g (3/5) contained vitellogenic follicles. Both water-borne exposure and feeding at 200 μg/g suppressed follicle activation or PG-PV transition. The numbers shown in each sample indicate total number of fish sampled (lower) and the number of fish that showed the same phenotype (upper). (B) Follicle distribution in bmp15-/- ovary after E2 treatments. The mutants could break the blockade at PV stage after treatment with E2 at 2 and 20 μg/g diet and their follicles could enter vitellogenic growth to reach the size range of EV stage (~ 350 μm).