Fig. 1

Fig. 1

hrh1 expression in Turku wt fishes and the mutation site of hrh1^−/− fish. a In situ hybridization of hrh1 in Turku wt zebrafish at 3 dpf (lateral and dorsal view), 5 dpf (lateral and dorsal view). Scale bars: 50 µm. b qRT-PCR results of hrh1 mRNA expression at 5 hpf, 10 hpf, 24 hpf, 3 dpf, 6 dpf, and 12 dpf. N = 6. Mean ± SEM. c The mutation site of hrh1^−/− zebrafish and genomic DNA sequencing results. d Predicated 3D modeling of hrh1^+/+ and hrh1^−/− protein by using SWISS-model online. e Brightfield image of larval zebrafish at 6 dpf. Scale bar: 200 µm.