Plasticity of cone transcripts in response to 5 days of T4 treatment. (A–F) Projections of representative whole, imaged retinas from fish treated with NaOH (control, A–C) or T4 (treated, D–F) followed by HCR in situ. Insets show indicated regions enlarged by 1400%. (A,D) All imaging channels merged. (B,E) lws2 (C,F) lws1. Note expanded lws1 domain and lack of lws2 mRNA in T4-treated retina. (G) qPCR of whole adult eyes. (lws1) n = 5, p = 0.0079 (Mann–Whitney). (lws2) n = 5, p = 1.2749E−05 (t-test). (gngt2b) n = 5, p = 0.0025 (t-test). (rh2-1) n = 4, p = 0.0168 (t-test). rh2-2) n = 5, p = 0.0355 (t-test). D dorsal, T temporal.
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