Figure 1

Figure 1

Topography of lws1 and lws2 reporter patterning in adult lws:PAC(H) transgenic fish, in which GFP reports lws1 expression and RFP reports lws2 expression. (A–F) Projections of representative whole, imaged retinas from fish treated with 0.01% NaOH (control, A–C) or 386 nM T4 in 0.1% NaOH (treated, D–F) for 5 days. Insets show indicated regions enlarged by 1400%. (A,D) All imaging channels merged. (B,E) lws2:RFP. (C,F) lws1:GFP. Note expanded GFP expression domain in response to T4 (D–F). (G,H) Projections of representative sectioned retinas from fish treated with NaOH (control, G–G″′) or T4 (treated, H–H″′). (G,H) All imaging channels merged. (G′,H′) lws2:RFP. (G″,H″) lws1:GFP. (G″′,H″′) enlarged image of lws2:RFP-containing region. Arrows indicate cells coexpressing GFP and RFP (see also Supplementary Fig. S1). (I–K) Analysis of areas of expression domains, n = 3 for each group. (I) Percent of retina occupied by GFP+ cells (p = 0.0068, t-test). (J) Percent of retina occupied by RFP+ cells (p = 0.6910, t-test). (K) Percent of retina occupied by GFP+ and RFP+ cells interspersed or coexpressing (p = 0.0033, t-test). Fisher’s Exact Test was used to test for overall differences: p = 0.0034 with a 3 × 2 contingency table. D dorsal, T temporal.